Abstract
The bioactivity of the entomopathogenic fungus, Isaria fumosorosea, and the plant secondary chemical, 20-Hydroxyecdysone, against Plutella xylostella were tested. 20-Hydroxyecdysone gave an apparent increase in adjusted larval mortality in a dose-dependent manner, whereas no influence of 20-Hydroxyecdysone was observed on conidial germination, mycelial growth and conidial production of I. fumosorosea. Furthermore, the synergism between 20-Hydroxyecdysone and I. fumosorosea was studied under laboratory and field cage conditions. Percent cumulated adjusted mortality of P. xylostella after the application of different concentrations of 20-Hydroxyecdysone and I. fumosorosea showed that the mortalities were directly related to the concentration of each component in the mixtures and their cumulative effect was evident for an extended period.
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