Abstract
A transmission study was conducted to identify and confirm the insect vectors of viral diseases of king chilli Capsicum chinense Jacquin, at the Virology Laboratory, Assam Agricultural University, Jorhat. The study reveals that Cucumber Mosaic Virus (CMV), Potato Virus Y (PVY) and Chilli Leaf Curl Virus (ChLCV) were the viral diseases transmitted by Aphis gossypii (Glover), Myzus persicae (Sulzer) and Bemisia tabaci (Gennadius), respectively. The detection of CMV and PVY were done through DAS-ELISA assay and ChLCV was done through PCR technique. The results showed that A. gossypii and M. persicae were successfully transmitting the CMV and PVY (40% each). DAS-ELISA revealed that 57.14% of the plant and 66.67% of A. gossypii samples were found to be positive to CMV. While, 42.17 and 60.00 % of PVY detection was observed from plant and M. persicae samples, respectively. B. tabaci also successfully transmitted ChLCV with 80% of the tested plant and the PCR results revealed that the primer pair ChLCV F1 and ChLCV R1 successfully yields 550bp at annealing temperature of 48oC from the infected plant as well as from B. tabaci samples and fail to detect from healthy plants, aphids, and leafhoppers samples.
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