Abstract
Purpose of the Study. To increase awareness about the patient with a negative skin test response and insect sting allergy and to determine the frequency and significance of negative skin test responses in patients with a history of systemic reactions to insect stings. Study Population. Subjects were recruited for insect sting challenge study using advertisements. Subjects with a history consistent with a systemic immunoglobulin E (IgE)-mediated allergy to insects were evaluated. Methods. Venom skin testing, serologic IgE venom testing, and insect sting challenges were conducted. Intradermal skin testing to venom extracts (ALK-Abello Labs, Copenhagen, Denmark) was conducted in a range from 0.001 μg/mL to 1.0 μg/mL. Venom-specific serum IgE was detected using radioallergosorbent testing (RAST) (on-site assay using precommercial …
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