Abstract

The binding of 24 derivatives of Cydia pomonella sex pheromone (dodecanol framework with no, one, or two conjugated double bonds at different positions of the aliphatic chain) and sodium dodecyl sulfate (SDS) to unmodified, acylated, alkylated, and esterified β-lactoglobulin (BLG) was studied by fluorescence spectrophotometry. Their apparent dissociation constants were in the range 0.2-0.9×10 -6 M (apparent molar ratio ca. I). Dodecyl acetate and SDS displayed the highest affinity for BLG, when (E,E)-6,8-dodecadienyl acetate and (E,E)-7,9-dodecadienyl acetate did not bind to BLG in the applied conditions. The apparent dissociation constants of derivatives unconstrained by conjugated double bonds did not differ

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