Insect cytokine, growth‐blocking peptide, is a primary regulator of melanin‐synthesis enzymes in armyworm larval cuticle

Abstract

The cuticles of most insect larvae have a variety of melanin patterns that function in the insects' interactions with their biotic and abiotic environments. Larvae of the armyworm Pseudaletia separata have black and white stripes running longitudinally along the body axis. This pattern is emphasized after the last larval molt by an increase in the contrast between the lines. We have previously shown that 3,4-dihydroxy-L-phenylalanine (Dopa) decarboxylase (DDC) is activated during the molt period by preferential enhancement of its transcription in the epidermal cells beneath the black stripes. This study demonstrated that tyrosine hydroxylase (TH) expression is activated synchronously with DDC. Furthermore, enhancement of DDC and TH transcription is due to an increase in cyotoplasmic Ca(2+), which is induced by the insect cytokine, growth-blocking peptide (GBP). Enhanced gene expression for both enzymes was induced by substitution of the calcium ionophore A23187, and completely blocked by EGTA. A GBP-induced increase in cytoplasmic Ca(2+) was seen in epidermal cells under the black stripes but not those beneath the white stripes, suggesting that a difference in Ca(2+) concentration in stripe cells leads to the specific expression of DDC and TH genes. Based on the fact that epidermal cells beneath the white stripes contain abundant granules composed mainly of uric acid, which can form a complex with Ca(2+) and hence decrease its free concentration, we discuss the possibility that uric acid, as well as GBP, contributes to the difference in cytoplasmic Ca(2+) within the epidermal cells.