Abstract
In modern conditions of intensification of agriculture the industrial animal breeding involves the use of antibacterial drugs aimed at the prevention of infectious diseases. However, uncontrolled use of antibiotics leads to the selection of antibiotic-resistant strains, it should be noted that the treatment of diseases caused by these strains is difficult. Insect peptides have a barrier function and are able to protect the macroorganism from various pathogenic agents. In this study the original method of isolating protein fractions from the biomass of insect G. mellonella and M. domestica larvae was developed. The 19 protein fractions (PF) were obtained and their antimicrobial activity was studied as related to S. aureus ATCC 6538 (209-P), S. typhimurium 1626, C. albicans РКПГY –401/NCTC–885-653, B. cereus ATCC 10702. It was found that protein fractions No. 2, 2.3, 2.6, 3.4, 3.6 had antimicrobial activity as related to S. aureus ATCC 6538 (209-P); protein fractions No. 2, 2.6, 3.2, 3.6 inhibited the growth of S. typhimurium 1626; protein fractions No. 2, 2.3, 2.6, 3.3, 3.6 had an inhibiting effect on C. albicans РКПГY-401/NCTC-885-653, PF 2, 2.6, 3.2, 3.6, 3.7 had antimicrobial activity as related to B. cereus ATCC 10702.
Highlights
Nowadays there are research investigations related to the discovery of antimicrobial peptides (AMPs), which are effective in the treatment of infections caused by microorganisms related to substances produced by insects
A small amount of phosphate buffer salt solution (FSB) was added to the samples to dissolve the precipitation before chromatographic separation and the solution was injected into the chromatograph
The antimicrobial activity of protein fractions No 2.1-2.7; 3.1-3.7 isolated from the biomass of G. mellonella larvae and PF No 1-6 isolated from the biomass of M. domestica as related to S. typhimurium 1626, S. aureus ATCC 6538 (209-P), C. albicans РКПГY -401/NCTC-885-653, B. cereus ATCC 10702 were determined by the macrotube method
Summary
Nowadays there are research investigations related to the discovery of antimicrobial peptides (AMPs), which are effective in the treatment of infections caused by microorganisms related to substances produced by insects. The reason for this is the evolutionary success that allows insects to occupy different habitats. There are more than 30 million species of insects, that is, a huge source of resources for finding new substances for further use in medicine and veterinary [1,2,3,4]. In vivo AMPs of different classes and various antimicrobial substances have a synergistic antimicrobial effect [5]. The development of resistance in microorganisms to antimicrobial peptides in vitro is unlikely [6]
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