Inputs from three brainstem sources to identified neurons of the mouse inferior colliculus slice

Abstract

A total of 40 neurons from of the central nucleus of the mouse inferior colliculus (IC) were recorded intracellularly from brain slices to determine input properties by electrical stimulation of the ipsilateral lateral lemniscus (LL), commissure of Probst (CP), and commissure of the IC (CoIC) together with cellular morphology (in 25 neurons) by biocytin injection and staining. Nine neurons had oriented (bipolar), 16 neurons non-oriented (multipolar) dendritic trees of various sizes. Axon collaterals of a given neuron often ran in several directions to provide multiple input to adjacent isofrequency laminae, the lateral nucleus of the IC, the brachium of the IC, the LL, the CP, and the IC commissure. Neurons were classified by spike response patterns to depolarizing current injection into onset- and sustained-spiking cells. The former had significantly shorter membrane-time constants, significantly less frequently and smaller hyperpolarizations after spike occurrence, and more Ca 2+-humps. These properties and their preferred position in the dorsolateral ICC suggest a participation in binaural temporal processing. Almost all oriented cells showed only excitatory post-synaptic potentials (EPSPs) after LL stimulation, while in non-oriented cells inhibitory post-synaptic potentials (IPSPs) after the EPSPs were significantly more frequent. Neurons with largest dendritic trees and many dorsalward projecting axon collaterals were found in the ventral IC. There, neurons had average 4 ms (two synapses) shorter response latencies to LL stimulation than dorsally located neurons. Thus, neurons in the central and dorsal IC may receive mono- and disynaptic input from ventrally located neurons.