Abstract
We present here the concept of “in-plant” NMR and show that high-resolution NMR spectroscopy is suitable for the analysis of intact plants and can be used to follow the changes in the intraorganismal molecular composition over long time periods. The NMR-based analysis of the effect of different concentrations of heavy water on the aquatic plant Vesicularia dubyana revealed that due to the presence of specific adaptive mechanisms this plant can sustain the presence of up to 85% of D2O. However, it dies in 100% heavy water.
Highlights
The application of the high-resolution NMR spectroscopy for the analysis of living biological objects represents an interesting direction in the modern biological research since it allows one to “spy” what happens at the molecular level during the life of the organism
Increase in the concentration of heavy water to 85% and incubation under these conditions for 4–5 days resulted in a steady decrease in the signal intensity of the aliphatic region of the NMR-spectrum and in the appearance of intense narrow signals, corresponding to formic acid (0.9 ppm) and acetate
To change the concentration of heavy water, the medium was gently extracted from the NMR tube, the calculated amount of D2O was added, and the adjusted medium was gently returned to the vial
Summary
The application of the high-resolution NMR spectroscopy for the analysis of living biological objects represents an interesting direction in the modern biological research since it allows one to “spy” what happens at the molecular level during the life of the organism. Java moss known as aquatic or aquarium moss (Vesicularia dubyana, Hypnacea, Bryopsida) as a model organism for the “in-plant” NMR analysis. The choice of this moss, which is one of the commonly used aquarium plants, as a subject of research was determined by its availability, ease of cultivation, extreme ruggedness, and the relative insensitivity of this plant to the cultivation conditions. We analyzed the specific time-dependent changes in the NMR spectra of the living plant cultivated under the variety of conditions over the prolonged period of time. On the other hand, deuterated or heavy water is considered as a preferential agent in NMR spectroscopy, since it does not introduce distortions into the spectra
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