Inotropic and chronotropic effects of ischemic postconditioning on the model of isolated heart

Abstract

The effects of global ischemia (45 min) and reperfusion (30 min) on left-ventricular developed pressure (LVDP), HR, and end-diastolic pressure were studied on isolated perfused rat heart. The following postconditioning protocols were used: 1) 3 cycles of reperfusion (10 sec) and ischemia (10 sec), total cycle duration 20 sec; 2) 6 cycles reperfusion of reperfusion (10 sec) and ischemia (10 sec), total cycle duration 20 sec; 3) 3 cycles of reperfusion (20 sec) and ischemia (20 sec), total cycle duration 40 sec; 4) 6 cycles of reperfusion (20 sec) and ischemia (20 sec), total cycle duration 40 sec; 5) 3 cycles of reperfusion (30 sec) and ischemia (30 sec), total cycle duration 60 sec. The use of several cycles of a total duration of 20 sec had no effect on LVDP, but reduced EDP throughout the reperfusion period. Postconditioning protocol consisting of three 40-sec cycles promoted LVDP recovery during the reperfusion, but had no effect on EDP and decelerated HP normalization. Six 40-sec cycles had no effect on LVDP and EDP, but impeded HR recovery during the reperfusion period. Postconditioning protocol consisting of three 60-sec cycles promoted LVDP increase during the reperfusion and reduced contracture, but these transient effects were accompanied by decelerated HP normalization.