Inositol phospholipids are probably not the source of arachidonic acid for eicosanoid synthesis in astrocytes

Abstract

In astrocyte-enriched cultures of the rat cerebral cortex the Ca 2+ ionophore A23187 provoked the breakdown of inositol phospholipids, the liberation of arachidonic acid and the release of prostaglandins E 2, F 2α, I 2 and thromboxane A 2. However, agonists for receptors also coupled to inositol phospholipid metabolism in these cells failed to produce an increase in the release of both arachidonic acid and eicosanoids. Results suggest that the A23187-stimulated release of arachidonic acid and eicosanoids is caused by a phospholipase A 2-mediated attack on lipids other than the inositol phospholipids. Moreover, receptors linked to inositol lipid turnover are not involved in the control of eicosanoid release from astrocytes.