Inositol Hexaphosphate (IP6): Modulation of Cell Cycle and Proliferation of Bladder Cancer in vitro

Abstract

Introduction: We hypothesized that inositol hexaphosphate (IP6) would modulate cell cycle and cellular growth in bladder cancer. Materials and Methods: Bladder cancer cell lines were treated with 2.5 or 4.5 mM/well IP6. Cell viability was measured by MTT at 24 and 48 h. Cell cycle analysis was measured by DNA staining and quantified by FACS analysis. Results: Cell growth was inhibited in the TCCSUP, HTB9 and T24 cells (p < 0.001) with the exception of the HTB9 cells at 24 h. G1 phase fractions increased in the TCCSUP and HTB9 cells, while S phase fraction was decreased (p < 0.001). G2 phase was decreased in the TCCSUP cells (p < 0.001). G1 phase fraction was decreased in the T24 line with 4.5 mMIP6 (p < 0.001), while a significant increase in the S phase was observed in the T24 cells (p < 0.001). Conclusions: These results indicate that IP6 is potentially a highly effective treatment for carcinoma of the bladder.