Abstract
1. 1. IMP dehydrogenase (EC 1.1.1.205) from porcine thymus glands has been purified to homogeneity. 2. 2. The enzyme has a subunit MW of 57 kDa and an amino acid composition similar to those obtained from other normal and cancerous mammalian cells. 3. 3. The apparent K m values at pH 8.0 for IMP and NAD + are 7 and 16 μM, respectively. 4. 4. GMP, XMP and AMP are competitive inhibitors towards IMP with K i values of 50, 85 and 282 μM, respectively. 5. 5. The effectiveness of nucleotides to protect inactivation by CI-IMP is IMP > GMP > XMP > AMP.
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