Inosine effects on plasma inorganic phosphate movements in fresh and cold-stored blood.

Abstract

SummaryMovements of P32O4 in and out of fresh and cold-stored human erythrocytes were measured in presence and absence of inosine. I. Small amounts of hemolysis when inosine was present caused a decrease in plasma inorganic phosphate and incorporation of P32 into plasma organic phosphate. This formation of plasma organic phosphate decreased movement of P32 into the cell. II. When P32 was originally present in the red cell, inosine decreased the net movement from cells to plasma. We attribute this effect to utilization of inosine as substrate in the cell. III. Cells containing P32 during 6-day cold-storage lost 3 to 4 times as much P32 as cells having P32 for a short time. Movements from cells to plasma are described as the result of at least 2 phosphate compartments within the cell.