Inorganic carbon uptake by phytoplankton in Vineyard Sound, Massachusetts. II. Comparative primary productivity and nutritional status of winter and summer assemblages

Abstract

Using time-course, natural-light incubations, we assessed the rate of carbon uptake at a range of light intensities, the effect of supplemental additions of nitrogen (as NH 4 + or urea) on light and dark carbon uptake, and the rates of uptake of NH 4 + and urea by phytoplankton from Vineyard Sound, Massachusetts from February through August 1982. During the winter, photoinhibition was severe, becoming manifested shortly after the start of an incubation, whereas during the summer, there was little to no evidence of photoinhibition during the first several hours after the start of an incubation. At light levels which were neither photoinhibiting nor light limiting, rates of carbon uptake normalized per liter were high and approximately equal during winter and summer (22–23 μg C·l −1 · h −1), and low during spring (<10 μg C· l −1· h −1). In contrast, on a chlorophyll a basis, rates of carbon fixation were as high during spring (15–20μg C·μg Chl a −1·h −1), when concentrations of chlorophyll a were at the yearly minimum (<0.5 μg · l −1) as during the summer, when chlorophyll a concentrations were substantially higher (0.8–1.3 μg · l −1). Highest rates of NH 4 + and urea uptake were observed during summer, and at no time of the year was there evidence for severe nitrogen deficiency, although moderate nitrogen nutritional stress was apparent during the summer months.