Abstract
The anaerobic treatment of methanolic wastewater has become a crucial issue. Sodium bicarbonate (NaHCO3), as a typical representative of inorganic carbon substances, not only provides alkalinity in the anaerobic treatment of methanol but also governs the competition between methanogens and acetogens. However, a suitable dosage has rarely been mentioned. In this study, we demonstrate the crucial role of NaHCO3 in the anaerobic treatment of methanol using a long-term operated up-flow anaerobic sludge blanket (UASB) reactor and batch tests. The UASB results revealed that by adding 3 to 5 g/L of NaHCO3 to the influent at a high organic loading rate of 50 gCOD/L/d, a significant accumulation of acetic acid was detected, with a maximum value of up to 1400 mg/L. In the context of microbial community evolution, this study profiled substrate competition between the acetogens Acetobacterium and Methanomethylovorans, which directly utilize methanol, as well as the synergistic effects of Acetobacterium and acetoclastic Methanosaeta. Abundance analysis of genes encoding relevant pathway enzymes also revealed that the acetic acid production pathway was significantly enhanced when more NaHCO3 was added. According to the dose–response mechanism in the batch test based on kinetic analysis, an acceptable threshold prediction scheme was beneficial in reasonably adding NaHCO3 to improve acid production capacity and rapid methanol degradation, and ensuring that the generated acetate could be rapidly degraded without inhibition, in the high-rate anaerobic treatment of methanolic wastewater.
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