Inorganic cadmium increases the frequency of chemically induced chromosome aberrations in cultured mammalian cells

Abstract

The co-clastogenic effect of cadmium ion (Cd 2+) was studied in Chinese hamster CHO K1 cells and excision repair-deficient human XP20SSV cells. Cd 2+at ≦ 28.0 μM did not show any clastogenic effects under the experimental conditions used. Cd 2+post-treatment at ≦ 3.50 μM, however, increased the number of both breakage- and exchange-type chromatid aberrations induced by mitomycin C (MMC) and 4-nitroquinoline 1-oxide (4NQO) in CHO K1 cells. Enhancement of chromosome aberrations induced by MMC was observed when CHO K1 cells were treated with Cd 2+ during the G1 phase. Cd 2+ was also co-clastogenic with MMC in XP20SSV cells. Its co-clastogenic effect, however, was not observed in 4NQO-treated XP20SSV cells. These results suggest that Cd 2+ inhibits DNA pre-replication repair, perhaps DNA excision repair, thereby causing co-clastogenic effects.