Inorganic apatite nanomaterial: Modified surface phenomena and its role in developing collagen based polymeric bio-composite (Coll-PLGA/HAp) for biological applications.

Abstract

Nano sized bio-composites containing inorganic particles conjugated with polymer and protein are considered as potential material for tissue engineering systems like bone repair and advanced drug delivery. More specifically, hydroxyapatite (HAp), a well known as the strong bioactive material has limitations on reactivity towards biological systems. Thus, this work explains the interaction betweena natural biomaterial Collagen and poly (lactide co-glycolide)-Hydroxyapatite (HAp) composite. PLGA/HAp composite was fabricated by in-situ polymerization of DL-lactide, glycolide and HAp nanoparticles. The prepared PLGA/HAp composite was examined for physico-chemical properties by FTIR, DSC, SEM, and DLS. The microscopic image confirms the positioning of a highly ordered structure containing Coll-PLGA/HAp that leads to enhancement in thermal stability of collagen. The nature of bonding and structural orientation of bio-composite was thoroughly investigated by FTIR and SEM. Toxicity of bio-composites on A549 human lung cancer cell line and L929 mouse normal cell line were analysed, and results showed a decreasing trend in the cell viability, on increasing the concentration of bio-composite. As an effective option for tissue engineering, the scaffold was prepared by vacuum drying method. Porosity and tensile strength measurements of scaffold reveal that non-toxic characteristics of bio-composite, excellent pore distribution of scaffold and thermal resistivity make it a versatile material for tissue engineering.