Abstract
BackgroundDiabetes mellitus is a systemic disease mainly caused by the disorder of metabolism, which has become huge threat to human health. Polysaccharides are the main active substance from Inonotus obliquus (I. obliquus) with hypoglycemic effect. This study aims to evaluate the hypoglycemic activity and investigate the molecular mechanism of I. obliquus polysaccharide (IOP) in streptozotocin (STZ)-induced diabetic mice using metabolomics based on UPLC-Q-Exactive-MS method.ResultsThe results showed that the oral administration of IOP in high dose (1.2 g/kg) can significantly reduce the blood glucose with 31% reduction comparing with the diabetic model and relieve dyslipidemia in diabetic mice. By UPLC-Q-Exactive-MS method and multivariate statistical analysis, a total of 15 differential metabolites were identified, including 4 up-regulated and 11 down-regulated biomarkers, of which l-tryptophan, l-leucine, uric acid, 12-HETE, arachidonic acid, PC(20:1(11Z)/14:1(9Z)) and SM(d18:0/24:1(15Z)) were exhibited an important variation, as the potential biomarkers in diabetes. Pathway analysis indicated that phenylalanine, tyrosine and tryptophan biosynthesis and arachidonic acid metabolism were prone to interference in diabetes. Moreover, leucine and proline were reversed and phytosphingosine was further reduced in diabetic mice under the intervention of IOP.ConclusionIOP has predominant hyperglycemic effect on STZ-induced diabetic mice via ameliorating serum profiling.
Highlights
Diabetes mellitus is rapidly becoming a global epidemic disease and brings huge threat to human health and social economy
Several authors have reported that I. obliquus polysaccharide (IOP) may exert its antioxidant effect in treating hyperglycemia [20,21,22], of which the dry matter of culture broth of I. obliquus in 500 and 1000 mg/kg body weight showed a significant decrease in blood glucose level in alloxan-induced diabetic mice
In this study, a metabolomics technique based on ultra high performance liquid chromatography (UPLC)-Q-Exactive-MS method was used to illustrate the underlying mechanism of pathophysiological process in diabetes and therapeutic effect of IOP
Summary
Diabetes mellitus is rapidly becoming a global epidemic disease and brings huge threat to human health and social economy. The I. obliquus was traditionally used as a folk medicine to treat gastrointestinal cancer, cardiovascular disease and diabetes in Russia, China, and Korea for many years [12,13,14]. It contains a variety of bioactive substances, including polysaccharides, triterpenes, phenolic acids and flavonoids [15,16,17]. This study aims to evaluate the hypoglycemic activity and investigate the molecular mechanism of I. obliquus polysaccharide (IOP) in streptozotocin (STZ)-induced diabetic mice using metabolomics based on UPLC-Q-Exactive-MS method
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