Inonotus obliquus 액체배양을 통한 균사체 및 다당체 생산을 위한 최적 배양공정 개발

Abstract

This study was carried out to investigate the optimum liquid culture conditions for the mycelium and the polysaccharide production on liquid culture of Inonotus obliquus. In flask culture, the production of maximum mycelium and polysaccharide were 9.23 g/L and 1.85 g/L, respectively. In bioreactor, with the culture conditions of agitation rate 200 rpm and aeration rate 1 vvm, the production of mycelium and polysaccharide were 14.17 g/L and 2.81 g/L at the 8SUPth/SUP day of cultivation, respectively. And the cultivation was operated with 300 rpm and 1 vvm up to the 4SUPth/SUP day of cultivation, since then, 200 rpm and 1 vvm, resulting in that the production of mycelium was 15.3 g/L at the 9th day and the polysaccharide production was 2.93 g/L at the 6th day of the cultivation. Furthermore, with the same agitation and aeration rates as the above during the cultivation, the addition of glucose being 20g/L of the final concentration was intermittently accomplished in order to increase the mycelium and the polysaccharide production. As a result, the addition of glucose at the 3SUPrd/SUP day of cultivation resulted in that the maximum mycelium and the polysaccharide production were 17.1 g/L and 3.4 g/L at the 7SUPth/SUP day of cultivation, respectively, indicating that the cultivation period was 4 days shorter than the cultivation of 11 days.