Inoculum for the cultivation of Methylosinus sporium

Abstract

This research assessed the effect of the amount of inoculum on the specific growth rate of Methylosinus sporium culture. Methanotrophs were inoculated in different quantities onto media of the same composition and volume. Seven different initial inoculum concentrations were obtained in duplicate. Samples were cultured for 31 hours, during which sampling and optical density measurements were carried out. In accordance with the fact that the optical density is directly proportional to the concentration of microorganisms in the sample, the specific growth rate was calculated. The research revealed an interval from 6.5 to 24 hours, during which the culture demonstrates a stable growth rate of 0.06 h-1, at an inoculation dose at which the optical density of the nutrient medium with the added inoculum is 0.030-0.121.