Abstract
Heavy metals are considered to be one of the main sources of soil contamination. In this study, three tolerant bacteria were isolated from the heavy metal-contaminated soil in mining area, and immobilized bacteria were constructed using corn straw as the carrier. The combined remediation effect of immobilized bacteria and alfalfa in pot experiments was explored in heavy metal-contaminated soil. Under heavy metal stress, inoculation with immobilized bacteria significantly promoted the growth of alfalfa, in which the dry weights of roots, stems, and leaves increased by 19.8, 6.89, and 14.6%, respectively (P < 0.05). Also, inoculation with immobilized bacteria improved the antioxidant capacity of plants and the activity of soil enzymes and improved soil quality (P < 0.05). Microbial-phytoremediation technology effectively reduced the heavy metal content in the soil, and can restore the soil contaminated by heavy metals. The results will help to further understand the mechanism of microbial inoculation to reduce the toxicity of heavy metals, and provide guidance for the cultivation of forage grasses in heavy metal-contaminated soils.
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