Inoculation of Erythrina brucei with plant-beneficial microbial consortia enhanced its growth and improved soil nitrogen and phosphorous status when applied as green manure

Abstract

Erythrina brucei has been applied as a green manure to improve soil fertility in southern Ethiopia. It has been nodulated by indigenous rhizobia. The objectives of this study were to evaluate the effects of E. brucei inoculation with microbial consortia consisted of Bradyrhizobium shewense, Acinetobacter soli and arbuscular mycorrhizal fungi (AMF)on E.brucei growth, soil nitrogen and phosphorous status after application as a green manure.A field experiment was conducted by inoculating E. Brucei with different microbial consortia. E. brucei inoculated with the microbial consortia were grown for 150 days. Its shoot length was measured at 60, 90, 120 and 150 days after planting. Then, plants were uprooted and mulched as a green manure. The soil nitrogen, available phosphorous and soil organic matter analysis were done. The experimental design was completely randomized block design with eight treatments comprised of three replications. Inoculated treatments did not show a significant (p < 0.05) difference in shoot length in the first 60 days. However, shoot length was increased between 19.1 and 41.3 %, 10.5–43.4 % and 8.7–37.6 %, respectively at 90, 120 and 150 days. The soil organic matter was improved in both inoculated and un-inoculated treatments. The improvements in the soil organic matter of un-inoculated treatments may be due to the decomposition of un-inoculated plants biomass in the soil. The B. shewense inoculation improved the soil nitrogen by 17 %. The soil phosphorous was improved in 57 % of inoculated treatments. The inoculation of E. brucei with microbial consortia enhanced its growth and improved soil fertility when applied as a green manure. Inoculating the green manure legumes with symbiotically effective rhizobia and plant-beneficial microbes can enhance the growth of E. brucei and its nutrient uptake.