Abstract
The drug L-DOPA has been widely used against Parkinson’s disease and is extracted from plants. Due to the increasing demand of this drug, new plant sources need to be discovered in addition to the existing sources. The paper embodies results on Mucuna monosperma, which can be a promising candidate for L-DOPA. The seed powder of this plant contains 5.48% of (dry weight) the drug and when the seeds were soaked in distilled water, content was increased to 6.58%. Different elicitors when added, enhanced the drug level in seed up to 11.8%. The possible rationale behind this increase was confirmed by increase in tyrosinase activity in the seeds. Presence of L-DOPA was confirmed using various analytical techniques as HPLC, HPTLC and NMR. The work demonstrates a potential candidate plant as a source for L-DOPA when a novel method was adopted as described here. Electronic Supplementary MaterialSupplementary material is available for this article at 10.1007/s13659-011-0051-3 and is accessible for authorized users.
Highlights
Parkinson’s disease (PD), a degenerative disease of the nervous system and is characterized by muscular rigidity, difficulty with balance and walking, depression and dementia.[1]
M. monosperma intact soaked seeds have been used for the extraction of L-DOPA, ; we have studied its synthesis as well as its enhancement by adding various activators
It gives an accurate value of L-DOPA content either in the seed powder or in the soaked seeds and can be an easy alternative to sophisticated instruments like High performance liquid chromatography (HPLC), when a slight loss of sensitivity can be compromised
Summary
Parkinson’s disease (PD), a degenerative disease of the nervous system and is characterized by muscular rigidity, difficulty with balance and walking, depression and dementia.[1]. We have used both intact seeds and seed powder, soaked intact seeds showed increased L-DOPA content because, soaking of the seeds in distilled water with elicitors leads to the activation of enzymes responsible for the synthesis of L-DOPA. The L-DOPA content in the M. monosperma seed powder when soaked in distilled water was found to be 4.75% (dry weight).
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