Abstract
We used light to irradiate skin-derived stem cells and tried to find any cellular protein alterations 24 h after illumination. A 266-nm laser with four intensities was used, and of the nine cell markers that were surveyed in our trials, only CD90 was downregulated at an intensity of 20 μJ for 10 s. Repeated illuminations from the 266-nm laser at seven intensities revealed that CD90 expression was downregulated 14.6–28.8%, depending on light intensity. The maximal effect was noted at an intensity of 30 μJ for 2 s. This innovative finding reveals that a 266-nm laser can regulate protein expression in skin-derivative stem cells.
Highlights
We used light to irradiate skin-derived stem cells and tried to find any cellular protein alterations 24 h after illumination
We report an innovative finding that 266-nm light could regulate the expression of CD90 in skin-derived stem cells (SDSCs)
The goal of those studies was to find a way to kill the cancer cells (HeLa cells), but the results disappointedly indicated that some cancer cells grew better than control, and some cancer cells showed increased DNA replication[7]
Summary
We used light to irradiate skin-derived stem cells and tried to find any cellular protein alterations 24 h after illumination. The maximal effect was noted at an intensity of 30 μJ for 2 s This innovative finding reveals that a 266-nm laser can regulate protein expression in skin-derivative stem cells. We surveyed the absorption spectrum of each nucleotide over the range of 240–290 nm (Fig. 1) This inspired us to use a 266-nm laser to irradiate skin-derived stem cells (SDSCs) and survey their responses. An increased DNA replication rate was noted in PPP-266 and HRRP-2717 The goal of those studies was to find a way to kill the cancer cells (HeLa cells), but the results disappointedly indicated that some cancer cells grew better than control, and some cancer cells showed increased DNA replication[7]. Karu’s purpose was to kill the cancer cells, but ours was to investigate how the 266-nm laser acted on protein expression of stem cells
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