Innovative design of a methodology for the simultaneous determination of compounds by kinetic-spectroscopy three-dimensional chemiluminescence.

Abstract

This report presents a novel methodology based in the quickly acquisition of full UV-vis range spectra combined with the joint determination of kinetic parameters and chemiluminescence signals. Then this technique allows obtaining three-dimensional chemiluminescence spectra profiles containing kinetic and spectroscopic information. That is especially useful for resolving mixtures of luminophors because of more analytical information for each analyte is available increasing the selectivity relative to conventional chemiluminescence methods. To accomplish this, a conventional Back-Thinned CCD detector is used and the three-dimensional chemiluminescence spectra is subsequently processed so that two-dimensional spectra with different trajectories can be obtained by dedicated software CLTotal. The potential of the proposed analytical methodology was assessed for the simultaneous determination of two polycyclic aromatic hydrocarbons. The chemiluminescent reaction between DNPO and hydrogen peroxide induced by their presence was used to record 3D spectra and the software CLTotal to subsequently construct linear variable-angle trajectories in the spectra, in order to obtain more selective 2D spectra facilitating the simultaneous determination of the analytes. The results of the statistical analysis testify to the usefulness of the proposed method for the intended purpose.