Innovative biofilm inhibition and anti-microbial behavior of molybdenum sulfide nanostructures generated by microwave-assisted solvothermal route

Nilam Qureshi,Valerio Causin,Rajendra Patil,Govind Umarji,Uttam Mulik,Wasudev Gade,Dinesh P Amalnerkar,Manish Shinde,Anand Bhalerao

doi:10.1007/s13204-014-0322-5

Abstract

The incessant use of antibiotics against infectious diseases has translated into a vicious circle of developing new antibiotic drug and its resistant strains in short period of time due to inherent nature of micro-organisms to alter their genes. Many researchers have been trying to formulate inorganic nanoparticles-based antiseptics that may be linked to broad-spectrum activity and far lower propensity to induce microbial resistance than antibiotics. The way-out approaches in this direction are nanomaterials based (1) bactericidal and (2) bacteriostatic activities. We, herein, present hitherto unreported observations on microbial abatement using non-cytotoxic molybdenum disulfide nanostructures (MSNs) which are synthesized using microwave assisted solvothermal route. Inhibition of biofilm formation using MSNs is a unique feature of our study. Furthermore, this study evinces antimicrobial mechanism of MSNs by reactive oxygen species (ROS) dependent generation of superoxide anion radical via disruption of cellular functions.

Highlights

Indiscriminate use of antibiotics against infectious diseases is turning out to be a vicious cycle of developing new antibiotic drug and its resistant strains in short time span due to inherent tendency of microbial cells to alter their genes
We present an expedient and ‘green’ microwave-assisted solvothermal synthesis (Shinde et al 2012b) of molybdenum disulfide nanostructures (MSNs) and hitherto unreported pre-therapeutic antimicrobial application protocol of the same
The synthesis of MSNs was carried out via microwaveassisted solvothermal technique reported for other materials (Shinde et al 2012a)

Summary

Introduction

Indiscriminate use of antibiotics against infectious diseases is turning out to be a vicious cycle of developing new antibiotic drug and its resistant strains in short time span due to inherent tendency of microbial cells to alter their genes. The synthesized products were explored for antimicrobial applications with two-pronged intentions: (1) to probe biofilm inhibition (in Psuedomonas aeruginosa 01) which is the earliest assertion of MSNs as anti-biofilm agents and (2) to assay the antibacterial property of synthesized nanoparticles (using model organisms, grampositive Bacillus subtilis NCIM 2063 and gram-negative Escherichia coli NCIM 2931 and corroborate the possible mechanism by detecting and measuring the reactive oxygen species (ROS) and simultaneously monitoring the redox enzymes.

Results

Conclusion