Abstract
Background The promise of pluripotent stem cells lies in their ability to form any cell or tissue in the body. However, this promise requires a stable and reproducible method to grow the cells. Current methods rely on feeder cells or extracellular matrix proteins to cover the cultureware growth surface, and either manual selection or enzymatic dissociation in cell passaging and harvesting. This study describes a novel and simple method to grow pluripotent stem cells without the use of feeder cells or extracellular matrix proteins.
Highlights
The promise of pluripotent stem cells lies in their ability to form any cell or tissue in the body
Human ESCs can be passaged a few times on the Nunclon Vita surface in mouse embryonic fibroblast (MEF)-conditioned media without Rho-kinase inhibition before the growth rate spontaneously declines
Human ESCs grown on the Nunclon Vita surface in the presence of Y-27632 have normal karyotype, express pluripotency markers (Fig. 1), and can be differentiated into embryoid bodies
Summary
The promise of pluripotent stem cells lies in their ability to form any cell or tissue in the body. This promise requires a stable and reproducible method to grow the cells. Current methods rely on feeder cells or extracellular matrix proteins to cover the cultureware growth surface, and either manual selection or enzymatic dissociation in cell passaging and harvesting. This study describes a novel and simple method to grow pluripotent stem cells without the use of feeder cells or extracellular matrix proteins
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