Abstract
ABSTRACT The course of the dorsal nerve in the fourth abdominal segment of the grasshopper Melanoplus differentialis is described. Unconventional techniques of making serial sections of the dorsal nerve for examination with the electron microscope are described in detail. The method permits a higher resolution than the light microscope but lower than more conventional electronmicroscope techniques. It has been demonstrated that the median dorsal internal muscles are all innervated from eight axons in the dorsal nerve. Although there is individual variation in the size of these axons there is some indication that they can be divided into four pairs according to their size. Of the remaining 13 axons in the dorsal nerve, two supply the median external dorsal muscle and 11 are concerned with the innervation of the heart.
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