Inner and inter population structure construction of Chinese Jiangsu Han population based on Y23 STR system.

Huipin Wang,Chun Yang,Huajie Ba,Jianqiu Zhang,Yunchun Tai,Tzen-Yuh Chiang

doi:10.1371/journal.pone.0180921

Abstract

In this study, we analyzed the genetic polymorphisms of 23 Y-STR loci from PowerPlex® Y23 system in 916 unrelated healthy male individuals from Chinese Jiangsu Han, and observed 912 different haplotypes including 908 unique haplotypes and 4 duplicate haplotypes. The haplotype diversity reached 0.99999 and the discrimination capacity and match probability were 0.9956 and 0.0011, respectively. The gene diversity values ranged from 0.3942 at DYS438 to 0.9607 at DYS385a/b. Population differentiation within 10 Jiangsu Han subpopulations were evaluated by RST values and visualized in Neighbor-Joining trees and Multi-Dimensional Scaling plots as well as population relationships between the Jiangsu Han population and other 18 Eastern Asian populations. Such results indicated that the 23 Y-STR loci were highly polymorphic in Jiangsu Han population and played crucial roles in forensic application as well as population genetics. For the first time, we reported the genetic diversity of male lineages in Jiangsu Han population at a high-resolution level of 23 Y-STR set and consequently contributed to familial searching, offender tracking, and anthropology analysis of Jiangsu Han population.

Highlights

Genetic markers derived from Y chromosome independent of recurrent mutations or recombination [1], play special roles in uncovering genetic structure [2,3] and inferring human dispersal and important migration time range [4,5]
Y-short tandem repeats (STRs) was typed for the PowerPlex1 Y23 System (Promega, USA) with GeneAmp PCR system 9700 (Thermofisher Scientific, Waltham, MA, USA)
As we have noted above, 916 unrelated healthy Han males from Jiangsu province were genotyped at 23 Y-STR loci. 912 different haplotypes, including 908 singletons (99.6%) and 4 duplicates (0.4%), were found in total comprising, revealing that the 23 Y-STRs contained in the PowerPlex1 Y23 System were highly polymorphic (HD = 0.9999952) in Jiangsu Han population and were great valuable for forensic application (DC = 0.9956, match probability (MP) = 0.0011)

Summary

Introduction

Genetic markers derived from Y chromosome independent of recurrent mutations or recombination [1], play special roles in uncovering genetic structure [2,3] and inferring human dispersal and important migration time range [4,5]. In Y chromosomes, genetic variations are inherited from father to son paternally. Y chromosome could reflect the gene flows and genetic differentiation of male lineages [6]. Genetic markers in Y chromosome have smaller effective population size compared to those in autosomes. The advancement of new sequencing technologies and more attention paid to human genome project provided abundant genetic markers in Y-DNA [3,7,8].

Methods

Findings

Discussion

Conclusion