Innate immune response of the dental pulp in healthy and carious human teeth

Ana Sotirovska Ivkovska,Zlatko Georgiev,Ljube Ivkovski,Efka Zabokova-Bilbilova,Emilija Bajraktarova Valjakova

doi:10.33320/maced.pharm.bull.2017.63.01.004

Ana Sotirovska Ivkovska, Zlatko Georgiev + Show 3 more

Open Access

https://doi.org/10.33320/maced.pharm.bull.2017.63.01.004

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Abstract

Antigen-presenting cells are capable of participating in the stimulation of T cells by antigen presentation. Antigen-presenting cells are considered essential for the induction and expansion of the immune reaction, because their interaction with antigen is the first step in immune induction. We have studied the distribution of class II-expressing cells in developing, healthy and carious human teeth to clarify when human pulp acquires an immunologic defense potential. Antigen-expressing cells were identified immunohistochemically with HLA-DR monoclonal antibodies (for dendritic cells) and CD68 monoclonal antibodies (for macrophages). In the pulp of unerupted developing teeth, HLA-DR-positive cells were distributed mainly in and around the odontoblast layer. A few CD68 positive cells were located more coronary around the blood vessels. In erupted teeth, HLA-DR-positive cells were located, for the most part just beneath the odontoblast layer. CD68 positive cells were also located coronary mainly around the blood vessels. Superficial caries lesions caused an aggregation of HLA-DR-positive cells and macrophages in the dental pulp corresponding to the lesion. Our results showed that human teeth are already equipped with an immunological defense potential prior to eruption. In the initial stage of caries infection, an immunoresponse mediated by class-II-expressing cells is initiated in human dental pulp. Keywords: human dental pulp, developing tooth, dental caries, dendritic cells, macrophages, immunohistochemistry

Highlights

Various types of immunocompetent cells are known to be present in normal dental pulp (Cox, 1992; Jontell et al, 1987, Jontell et al, 1992, Okiji et al, 1992).Antigen-presenting cells are considered essential forA
The purpose of this study was to elucidate the distribution of class II-expressing cells in developing, healthy and carious human teeth, to clarify when human pulp acquires an immunologic defense potential and how this reacts to dental caries
The HLA-DR-positive cells observed throughout the odontoblast layer are dendritic cells, and their location is ideal for capturing antigens invading the dental tubules (Bachereau et al, 2001; Reschigno et al, 2001)

Summary

Introduction

Various types of immunocompetent cells are known to be present in normal dental pulp (Cox, 1992; Jontell et al, 1987, Jontell et al, 1992, Okiji et al, 1992).Antigen-presenting cells are considered essential forA.

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