Abstract
Base‐pairing stability in DNA‐gold nanoparticle (DNA‐AuNP) multimers along with their dynamics under different electron beam intensities was investigated with in‐liquid transmission electron microscopy (in‐liquid TEM) using custom developed silicon nitride based liquid cells. Multimer formation was triggered by hybridization of DNA oligonucleotides to another DNA strand (Hyb‐DNA) related to the concept of DNA origami. We analyzed the degree of multimer formation for a number of samples and a series of control samples to determine the specificity of the multimerization during the TEM imaging. DNA‐AuNPs with Hyb‐DNA showed an interactive motion and assembly into 1D structures once the electron beam intensity exceeds a threshold value. These findings indicate that DNA base pairing interactions are the driving force for in situ multimerization and DNA‐metallic NP conjugates provide excellent models to understand structure‐function correlation in biological systems with nanometer spatial resolution (Keskin et al., 2015, 10.1021/acs.jpclett.5b02075).
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