Abstract
BackgroundThis experimental study was designed to investigate the differences in pattern of local growth and diffusion of colorectal cancer cells injected into either mesenteric (M) or antimesenteric (AM) sides of the colon.MethodsA total of 1 × 106 colonic adenocarcinoma cells (line DHD/K12-TRb) were injected into the cecal wall of BDIX syngeneic male rats at an M or AM site of the colon. At six weeks after injection, all animals were sacrificed and the presence or absence of tumor in the cecum as well as regional metastasis and peritoneal carcinomatosis were determined.ResultsSix weeks after injection, macroscopic tumor growth was observed in 27/37 (72%) animals in group M and 21/32 (65%) in group AM (P = 0.98). In group AM, diffuse peritoneal carcinomatosis was present in 19/21 rats (90.4%) versus 3/27 rats (11%) in group M; this difference was statistically significant (P = 0.025). Regional mesenteric lymph nodes were the only location in which tumor was detected in 23/27 rats (85%) in group M versus 2/21 (9.5%) in group AM; this difference too was statistically significant (P = 0.031)ConclusionThe patterns of diffusion of tumors implanted in mesenteric and antimesenteric sites of the colon appear to be different, although the reason for this is not clear.
Highlights
This experimental study was designed to investigate the differences in pattern of local growth and diffusion of colorectal cancer cells injected into either mesenteric (M) or antimesenteric (AM) sides of the colon
Several experimental models have been developed to examine colorectal cancer kinetics, but most are based on heterotopic cell implantation [3,4] both in nude and syngeneic rats [5,6]
In group AM, diffuse peritoneal carcinomatosis (Figure 3) was present in 19/21 rats (90.4%) versus 3/27 cases (11%) in group M (Figure 4); this difference was statistically significant (P = 0.025)
Summary
This experimental study was designed to investigate the differences in pattern of local growth and diffusion of colorectal cancer cells injected into either mesenteric (M) or antimesenteric (AM) sides of the colon. Identifying the pattern of cancer spread (local and distant) may be important in choosing the appropriate surgical and medical, strategies in relation to various factors, such as tumor location and site of growth. Other investigators have developed orthotopic models in which tumor implantation is performed directly into the wall of the colon of healthy rats, in order to study patterns of local growth and metastatic spread [7,8]. The present experimental study was designed to investigate the differences in pattern of local growth and diffusion of colorectal cancer cells injected into either mesenteric (M) or antimesenteric (AM) sides of the colon Several experimental models have been developed to examine colorectal cancer kinetics, but most are based on heterotopic cell implantation (i.e. peritoneal, subcutaneous, etc.) [3,4] both in nude and syngeneic rats [5,6].
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