Injection Molding of Cell Membrane Perforator with Photochemical Perforation Function and Surface Modification

Abstract

Recently, controlling cell function has attracted significant attention, with the possibility of changing various forms of cells and organs. Cell modification technology operates through various methods, such as the virus vector and lipofection methods; however, these approaches have problems in terms of efficiency and process optimization. In contrast, our photochemical cell membrane perforation method exhibits high efficiency, but requires a thermosetting silicone polymer-based cell membrane perforator as a consumable item, which requires 4 h for production. In this study, we present a process using an injection molding machine for manufacturing thermoplastic elastomer-based perforators for mass production, successfully shortening the production time to < 1 h while preserving the perforating function.