Abstract
From the peripheral leukocytes of healthy persons, 81 cell lines were initiated from 796 cultures, each containing an initial cell population of about 2 million lymphocytes. The proportion of cultures, prepared from a single blood sample, capable of yielding cell lines varied from 0-100%. Factors affecting this initiation of cell lines appeared to be the source of leukocytes and the method of cultivation. For some blood samples, the size of the sample may also be important. Subjected to routine 1 to 2 culture division every 3 or 4 days, many cell lines degenerated after a varying number of subcultures. Some degenerated as early as the third, others as late as the 104th, and still others were growing actively at the 143d subculture. By adjustment of the interval between culture divisions to the rate of cell multiplication, the in vitro lifespan of 3 or 4 lines was prolonged by at least 50 serial divisions. No cell line was initiated from 418 umbilical cord blood leukocyte cultures prepared from 44 samples. This finding suggests that cord blood is the ideal source of leukocytes for study of the growth-transforming capability of potential oncogenic substances.
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