Abstract
Abstract The situation of native orchids from natural environments becomes more vulnerable each day, especially those not protected by conservation units. Thus, studies on the propagation of species, especially on in vitro germination and initial establishment, are necessary for maintaining species in their habitat and reintroduction in restoration areas. This study verifies the influence of the nutritional composition of the culture media on in vitro germination and initial establishment of Miltonia flavescens. Seeds from natural pollination were sown in four different culture media and put in a growing room under controlled photoperiods and temperature. The germination was evaluated 30 days after sowing, and the initial growth of the seedlings at 120 and 180 days after sowing. The results indicated the Murashige and Skoog medium, complete and with half of the salt concentrations, promoted a higher germination percentage and were more effective in the initial development of M. flavescens seedlings.
Highlights
AND OBJECTIVESThe Orchidaceae family is widely distributed in Brazil with 2,475 species distributed among 220 genera, with confirmed occurrences in 27 states in the North, Northeast, Midwest, Southeast and South regions (JBRJ, [2018?])
The Knudson C (KC) medium formulated by Knudson in 1946, VW, established by Vacin and Went in 1949, and the MS culture medium developed by Murashige and Skoog in 1962 are some of the most used for in vitro cultivation of orchids (Silva et al, 2015; Suzuki et al, 2010; Vudala & Ribas, 2017)
Mature fruits of Miltonia flavescens Lindl. from natural pollination were used as the study material (Figure 1), from 12-year-old plants set in trees located in the Ornamental Plants Cultivation Area of the Universidade Federal da Grande Dourados – Faculty of Agrarian Sciences (UFGD/FCA)
Summary
The Orchidaceae family is widely distributed in Brazil with 2,475 species distributed among 220 genera, with confirmed occurrences in 27 states in the North, Northeast, Midwest, Southeast and South regions (JBRJ, [2018?]). Several species of this botanical family have medicinal and pharmacological properties and have being used for centuries in the traditional Chinese medicine (Lam et al, 2015; Silva et al, 2015; Tsering et al, 2017). The culture medium formulation is essential for the seed, since it provides the necessary constituents (minerals, vitamins, growth regulators, among others) for their development, and it can be composed of different combinations according to the requirements of each species (Silva et al, 2017)
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