Abstract
Non-obstructive azoospermia (NOA) is a cause of male infertility secondary to genetically driven defects in spermatogenesis. Testicular sperm extraction (TESE) is successful in identifying a small number of sperm in 50% of men with NOA. Traditionally, sperm are isolated from testicular tissue using a combination of standard light microscopy, enzymatic digestion and significant time by embryologists analyzing the specimens in hope to isolate rare spermatozoa. Here we discuss our preliminary results utilizing fluorescence-activated cell sorting (FACS) of testis tissue to increase the efficiency in the isolation of spermatozoa.
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