Abstract
Na +, K +-ATPase activity of homogenates prepared from cauda epididymal golden hamster sperm increased after the addition of cGMP (50μM), monobutyryl cGMP (0.5μM) or cGMP-dependent protein kinase (0.94μg/ml). Addition of monobutyryl cAMP (0.5μM) or purified catalytic subunit of cAMP-dependent protein kinase (1.26μg/ml) inhibited the activity of the Na +, K +-ATPase. Preincubation with a partially purified preparation of cAMP-dependent protein kinase inhibitor (75μg/ml) stimulated the activity of the Na +, K +-ATPase, and this stimulation was decreased by the addition of 5μM monobutyryl cAMP. It is not yet known whether direct and/or indirect mechanisms are involved, but these results are the first to describe such opposing effects by cyclic nucleotide-mediated processes on a Na +, K +-ATPase activity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
