Abstract
Objectives: To inititally evaluate the efficiency of cryoprotectant-free vitrification in PCR tubes on a low number of human spermatozoa. Methods: A laboratory experimental study was conducted on 30 severe oligospermia treated at the Military Institute of Clinical Embryology and Histology, Vietnam Military Medical University. The semen samples were vitrified and thawed at the following times, then sperm concentration, morphology, viability, and motility were evaluated after thawing. Results: The viable CSF, motile CSF when vitrifing without using CPAs were 50.45 ± 4.63; 43.35 ± 4.81 (1-week storage), 45.24 ± 4.33; 37.24 ± 4.39 (4-week storage) and 40.69 ± 3.96; 33.96 ± 4.14 (8-week storage), respectively. Conclusion: Vitrification of human sperm can be achieved without cryoprotectants and could be recommended for routine assisted reproductive technology.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
