Initial adhesion behavior of osteoblast onto Zr-1Cr alloy

Abstract

Event Abstract Back to Event Initial adhesion behavior of osteoblast onto Zr-1Cr alloy Satoshi Migita1*, Kunitaka Araki1, So Okuyama1 and Su Yalatu2 1 Yamagata University, Graduate School of Science and Engineering, Japan 2 Osaka University, Graduate School of Engineering, Japan Introduction: Zirconium (Zr) has a lower cytotoxicity, and high corrosion resistance due to passive oxide formation on its surface. However, the mechanical reliability of unalloyed Zr is insufficient for use as structural biomaterials. We employed Cr, as an alloying element, and created Zr1Cr alloy. In this study, we investigated an initial adhesion behavior of osteoblast onto the alloy. The cells were observed by fluorescent microscope, and the cell shape were processed through imaging analysis. Materials and Methods: Zr1Cr alloy was prepared by arc melting furnace and cast into the mold. After molding, Z1Cr processed to disk (6 mm in diameter × 2 mm in thickness). The disks were mirror-polished and rinsed for cell culture. Pure Zr purchased from Nilaco Corp., Tokyo, Japan. Protein adsorption property of Zr1Cr was analyzed using micro BCA-based colorimetric detection method. Bovine serum albumin (BSA; Wako, Tokyo, Japan ) was used as a model protein. A mouse osteoblast-like cell, MC3T3-E1 (RIKEN BioResource Center, Ibaraki, Japan) were cultured in minimum essential medium Eagle, alpha modification (alpha-MEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (JRH Biosciences, Inc., Lenexa, KS, USA) and antibiotic-antimycotic (Invitrogen). To evaluate initial adhesion behavior, cell attachment and spreading was investigated following the cells cultured on the Zr1Cr disks for 4 h. Results and Discussion: The amount of protein adsorption on Zr1Cr alloy was statistically greater than that on Zr. This result reflected relative permittivity of passive oxide. Osteoblast was cultured on each disks for 4 h. An adhesion activity of osteoblast on Zr1Cr alloy was similar to Zr. Thus, cytotoxicity of Zr1Cr was low. However, cell morphology was dramatically changed on Zr1Cr compared to Zr. Cells cultured on Zr1Cr was showed smaller adhesive area than that on Zr surface (Figure 1). Zr1Cr surface appeared to inhibit the elongation of the cells. In the 24 h incubation, almost cells were detached from Zr1Cr surface. Conclusion: In this study, we investigated initial adhesion behavior onto Zr1Cr alloy. The alloy was showed lower cytotoxicity, however, Cr, as an alloying element, appeared to inhibit a cell adhesion and elongation. The present data provide a basis for development of a technique for controlling of tissue compatibility of materials surface. Keywords: biomaterial, Implant, Biocompatibility, tissue compatibility Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Metallic biomaterials and alloys Citation: Migita S, Araki K, Okuyama S and Yalatu S (2016). Initial adhesion behavior of osteoblast onto Zr-1Cr alloy. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01511 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. * Correspondence: Dr. Satoshi Migita, Yamagata University, Graduate School of Science and Engineering, Yonezawa, Yamagata, Japan, Email1 Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Satoshi Migita Kunitaka Araki So Okuyama Su Yalatu Google Satoshi Migita Kunitaka Araki So Okuyama Su Yalatu Google Scholar Satoshi Migita Kunitaka Araki So Okuyama Su Yalatu PubMed Satoshi Migita Kunitaka Araki So Okuyama Su Yalatu Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.