Inhibotory effect of cyclic hexadepsipeptides on the protiferation activity of human PC-3 cells and the role of Sal-like gene 4

Abstract

Objective To investigate the inhibitory effects of cyclic hexadepsipeptides on human prostate cancer cells and the role of Sal-like gene 4 (SALL4).Methods Methyl thiazol tetrazolium (MTT) assay was used to detect proliferation rate of prostate cancer cells.Flow cytometry was used to detect apoptosis rate of prostate cancer cells.Western blotting was used to detect the Caspase-3,B cell lymphoma/leukemia-2 associated X protein (bax),B cell lymphoma/leukemia-2 (bcl-2) protein levels of prostate cancer cells.Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the SALL4 expression of prostate cancer cells.Results The survival rate of prostate cancer cells in cyclic hexadepsipeptides intervention after 24 h and 48 h was decreased [(32.40 ± 1.98) ％ and (38.20 ± 2.13) ％],and apoptosis rate increased [(48.43 ± 3.08) ％ and (50.08 ± 4.14) ％] as compared with the control group (P ＜0.01).The Caspase-3 (0.387 ±0.012) and bax protein (0.312 ±0.028) levels of prostate cancer cells in cyclic hexadepsipeptides intervention was increased after 24 h and 48 h as compared with the control group (P ＜ 0.05).The bcl-2 protein levels (0.312 ± 0.028) were decreased in cyclic hexadepsipeptides intervention group as compared with the C group (P ＜ 0.01),and the SALL4 had no significant change.Conclusion Cyclic hexadepsipeptides exert an inhibitory effect on the protiferation of prostate cancer cells probably by regulating Caspase-3,bax and bcl-2 levels. Key words: Prostate cancer; Cyclic hexadepsipeptides ; Sal-like gene 4 ; Caspase-3