Inhibitory of EV-A71 virus-induced apoptosis by ZVAD through ROS mediated signaling pathways

Tiantian Xu,Lu Kuang,Min Guo,Yi Chen,Bing Zhu,Yinghua Li,Haiyang Chen,Ruilin Zheng,Changbing Wang,Mingqi Zhao,Danyang Chen

doi:10.32604/biocell.2022.017757

Abstract

Emerging evidence that Enterovirus A 71 (EV-A71) infection closely related to apoptosis. The ZVAD is a caspase inhibitor that can prevent apoptosis. The aims of this project were to evaluate the mechanism of the ZVAD inhibited EV-A71 virus and to provide experiment basis for finding new antiviral drugs. In this study, after treated with ZVAD in EV-A71 infected Vero cells, the viral replication was reduced, and the cell viability was higher than EV-A71 group. Additionally, ZVAD decreased the cell apoptosis and the level of inflammatory cytokines induced by EV-A71 in the infected Vero cells. ZVAD inhibited cell apoptosis by regulating ROS mediated signaling pathway and inflammation cytokines to achieve antiviral.

Highlights

Hand, foot, and mouth disease (HFMD) is commonly affects children under the age of five in children (Li et al, 2017b; Zeng et al, 2019)
We evaluated the antiviral activities of ZVAD (25 μmol/L) in Vero cells by Cell Counting Kit-8 (CCK8) assay
ZVAD reduced the RNA and protein synthesis of Enterovirus A 71 (EV-A71) in Vero To further investigate the effects of ZVAD on the virus, the RNA and protein synthesis of EV-A71 were detected by Quantitative Real-Time PCR (QRT-PCR) and Western blot respectively

Summary

Introduction

Foot, and mouth disease (HFMD) is commonly affects children under the age of five in children (Li et al, 2017b; Zeng et al, 2019). HFMD is mainly caused by enterovirus A71 (EVA71), coxsackievirus 16 (Cox16), CV-A6 and CV-A10. Most of these infections are exhibit self-limiting characterized by skin rash and sores on hands, feet and in the mouth with or without fever. Some patients infected with EVA71 virus can develop severe central nervous system damage or cardiopulmonary complications or die (Cao et al, 2019; Li et al, 2019). It was reported that ZVAD can reduced apoptosis in virus infected cells. That was remained unclear how ZVAD modulate the antiviral activity in Vero cells

Objectives

Methods

Results

Conclusion