Inhibitory Effects ofChung Hun Wha Dam Tang(CHWDT) on High-Fat Diet-Induced Obesity via AMP-Activated Protein Kinase Activation

Md Jamal Uddin,Hun Taeg Chung,Min Zheng,Yeonsoo Joe,Hoyoung Lee,Tae-Oh Kwon,Sena Kim

doi:10.1155/2012/652473

Abstract

The Chung Hun Wha Dam Tang (CHWDT) herbal combination was reported to cease dizziness and phlegm. However, the effect of CHWDT in obesity has not yet been known mechanically. Therefore, we investigated whether this CHWDT could protect the cells from lipogenesis, gluconeogenesis, and inflammation in both in vivo and in vitro. CHWDT significantly decreased body weight, epididymal and perirenal fat content without affecting feed intake in high-fat diet-induced obese mice model. Additionally, CHWDT inhibited obesity-induced SREBP1, FAS, PGC1α, G6Pase, PEPCK and increased CPT1, ACO, and LCAD genes expression in vivo and in vitro. Proinflammatory cytokines like TNF-α and iNOS expression were reduced by CHWDT in both Raw264.7 macrophages and HepG2 cells. In addition, NO production was also significantly decreased by CHWDT in LPS-stimulated macrophages. Furthermore, AMPKα activation by CHWDT was involved in inhibition of obesity by reducing triglycerides production and increasing CPT1 expression. Based on all of the results, we suggest that CHWDT has inhibitory effects on obesity-induced lipogenesis, gluconeogenesis, and inflammation via AMPKα activation.

Highlights

In recent years, obesity is the most common metabolic disease emerging as a global problem especially in developed nations
Recent evidence shows that treatment with resveratrol, a polyphenolic compound enriched in grapes and red wine, ameliorates elevated levels of tumor necrosis factor (TNF)α, interleukin (IL)-6, and cyclooxygenase-2 in experimental diabetic neuropathy [7]
Obesity was induced by feeding the normal mice with high fat diet for 7 weeks while body weight of obese mice was 20% higher than mice fed with normal diet

Summary

Introduction

Obesity is the most common metabolic disease emerging as a global problem especially in developed nations. Obesity is closely associated with life-style-related diseases such as atherosclerosis and noninsulin-dependent diabetes mellitus and with increased risk of coronary heart disease [1] It is characterized by the activation of an inflammatory process in metabolically active sites such as adipose tissue, liver, and immune cells [2]. Recent evidence shows that treatment with resveratrol, a polyphenolic compound enriched in grapes and red wine, ameliorates elevated levels of tumor necrosis factor (TNF)α, interleukin (IL)-6, and cyclooxygenase-2 in experimental diabetic neuropathy [7] Lipolytic genes such as carnitine palmitoyltransferase I (CPT1), long chain acyl CoA dehydrogenase (LCAD), and acyl-CoA oxidase (ACO) are regulated in obese condition [8].

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Conclusion