Abstract
In this study, we evaluated the ability of Scutellaria baicalensis Georgi to protect lipid-peroxidation (LPO) in lung tissue after free radical-induced injury. We prepared S. baicalensis root (SBR) extracts using different solvents. The total flavonoid and total phenol contents of each extract were measured, and the ROS damage protection was evaluated by analyzing linoleic acid hydroperoxide (LHP)-induced LPO in rat lung mitochondria. Moreover, evaluating diphenylpicrylhydrazyl (DPPH), hydrogen peroxide, superoxide anion radical, and hydroxyl radical scavenging abilities and using metal chelating assays were used to determine in vitro antioxidant activity. The ethyl acetate (EtOAc) extract showed high ROS scavenging ability, and four compounds were subsequently isolated and purified from this extract: baicalin, baicalein, wogonin, and oroxylin A. Baicalein in rat lung mitochondria the most significant LHP-induced LPO inhibition was shown and extracted with EtOAc that contained the highest amount of baicalein. Thus, baicalein and the EtOAc extract of SBR may be efficient in conferring ROS damage protection and inhibiting LHP-induced LPO in rat lung mitochondria. Additional studies are warranted to investigate their use as antioxidant therapy for respiration infections, nutrition supplements, and lead compounds in pharmaceuticals.
Highlights
Numerous epidemiological investigations have highlighted the association between air pollution and respiratory diseases in recent decades, n; for example, pollutants exposure can cause reduction of lung function, chronic obstructive pulmonary disease (COPD), asthma, and cough [1]
We evaluated the ability of S. baicalensis root (SBR) extracts to protect the lungs from damage that is caused by LPO and free radicals
We examined the inhibition of lipid peroxidation in rat lung mitochondria using linoleic acid hydroperoxide (LHP) as inducer, which is closer to the real conditions of lung in vivo
Summary
Numerous epidemiological investigations have highlighted the association between air pollution and respiratory diseases in recent decades, n; for example, pollutants exposure can cause reduction of lung function, chronic obstructive pulmonary disease (COPD), asthma, and cough [1]. The number of deaths attributable to ambient air pollution exposure has been estimated to be 3.7–4.8 million, representing 7.6% of the total global mortality, according to a study that was conducted by. Health Organization (WHO), 29% of all deaths and deaths from lung cancer and 43% of all deaths and deaths from COPD are attributed to atmospheric particulate matter exposure and they are independent of cigarette smoking [4]. In 2014, >6000 deaths were attributed to ischemic heart disease, stroke, lung cancer, and COPD in Taiwan [5].
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