Abstract
This study was conducted to investigate the inhibitory effect of Lactobacillus cells and supernatants on the growth of the human colon cancer cell line HT-29. Our study results indicated that the PM153 strain exhibits the best adhesion ability and the highest survival in the gastrointestinal tract simulation experiment. Furthermore, after an 8-h co-culture of PM153 and HT-29 cells, the PM153 strain can induce the secretion of nitric oxide from the HT-29 cells. In addition, after the co-culture of the BCRC17010 strain (109 cfu/mL) and HT-29 cells, the Bax/Bcl-2 ratio in the HT-29 cells was 1.19, which showed a significant difference from the other control and LAB groups (p < 0.05), which therefore led to the inference that the BCRC17010 strain exerts a pro-apoptotic effect on the HT-29 cells. Upon co-culture with HT-29 cells for 4, 8 and 12 h, the BCRC14625 strain (109 cfu/mL) demonstrated a significant increase in lactate dehydrogenase (LDH) activity (p < 0.05), causing harm to the HT-29 cell membrane; further, after an 8-h co-culture with the HT-29 cells, it induced the secretion of nitric oxide (NO) from the HT-29 cells. Some lactic acid bacteria (LAB) strains have ability to inhibit the growth of the colorectal cancer cell line HT-29 Bax/Bcl-2 pathway or NO production. In summary, we demonstrated that the BCRC17010 strain, good abilities of adhesion and increased LDH release, was the best probiotic potential for inhibition of HT-29 growth amongst the seven LAB strains tested in vitro.
Highlights
IntroductionColorectal cancer is the second leading cause of death due to cancer in the United States [1]
Colorectal cancer is the second leading cause of death due to cancer in the United States [1].The incidence rate of colorectal cancer increases with age
PM177, PM153, BCRC17010 and BCRC14759 were maintained above 1088 cfu/mL, indicating fairly of PM177, PM153, BCRC17010 and BCRC14759 were maintained above 10 cfu/mL, indicating fairly good acid tolerance
Summary
Colorectal cancer is the second leading cause of death due to cancer in the United States [1]. The incidence rate of colorectal cancer increases with age. Excessive alcohol consumption may lead to an increase in the colorectal cancer incidence rate by 60%; insufficient fibre intake and smoking may increase the colorectal cancer incidence rate by 20%. Previous research has found that the excessive intake of high-fat diets can lead to colorectal cancer [2]. At least 15%–20% of cancers are attributed to infection or inflammation, including malignant tumours such as colorectal cancer and inflammatory bowel disease (including ulcerative colitis and Crohn’s disease). Research has suggested that patients with the above infections are more likely to develop colorectal cancer [3]
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