Inhibitory Effects of Natural Food Dyes Genistein on Invasion of SKOV3 Human Ovarian Carcinoma Cells <i>In Vivo</i> and <i>In Vitro</i>

Abstract

The invasion behavior of tumor cells is important in the metastasis process of ovarian cancer cells. In this study we investigated the effects of Genistein on invasion inhibition in ovarian carcinoma cell line SKOV3 in vivo and in vitro. The abilities of the Genistein-treated SKOV3 cells to invade through reconstitute matrigel in transwell chambers were investigated in vitro and the invasion effect in vivo was determined by using the xenograft models of SKOV3 in nude mice. The ability of the 20μmol/L Genistein-treated cells to invade the reconstitute basement membrane was decreased significantly at 72h. This inhibition was dose-dependent. 40μmol/L Genistein had the strongest effect. The in vivo result suggested that the grade of invasion in control SKOV3 cells was time-dependent and Genistein-treated group could apparently inhibit the progress of invasion, localizing the tumor in invasion Grade 0 or Grade I and decreasing the proportion of Grade II, III and IV. The results suggested that Genistein possessed inhibitory effect on invasion in human ovarian carcinoma cell lines SKOV3 in vivo and in vitro.