Inhibitory Effects of J78, a Newly Synthesized 1,4-Naphthoquinone Derivative, on Experimental Thrombosis and Platelet Aggregation

Abstract

Several compounds with the backbone of 1,4-naphthoquinone chemical structure have been reported to display antiplatelet and antithrombotic activities, indicating that this congener compound may be a new source in the antithrombotic drug development. In the present study, the possible antiplatelet activity and antithrombotic efficacy of J78 (2-chloro-3-[2′-bromo, 4′-fluoro- phenyl]-amino-8-hydroxy-1,4-naphthoquinone), a newly synthesized 1,4-naphthoquinone derivative, were examined. Orally administered J78 (50, 100 mg/kg) dose dependently protected mice against the collagen + epinephrine-induced thromboembolic death. Orally administered J78 also significantly inhibited the ADP- and collagen-induced rat platelet aggregation ex vivo, with inhibition values of 44 and 40%, respectively. J78 inhibited the collagen-, arachidonic acid- and thrombin-induced human platelet aggregation concentration dependently in vitro, with IC₅₀ values of 7.8 ± 0.4, 10.1 ± 0.4 and 18.4 ± 2.0 µmol/l, respectively. It was also active in inhibiting Ca²⁺ ionophore, A23187-induced platelet aggregation, suggesting that J78 may have an inhibitory effect on Ca²⁺ mobilization. J78, however, did not alter coagulation parameters such as activated partial thromboplastin time and prothrombin time in human plasma. Taken together, these results suggest that J78 may be a promising antithrombotic agent, and its antithrombotic activity may be due to antiplatelet rather than anticoagulation activity.