Abstract
Objective: To investigate the inhibitory effect of interferon-α (IFN-α) and curcumin on proliferation of Raji cells (B-NHL) and its mechanism. Methods: The morphological changes of Raji cells were observed in culture medium with IFN-α (500, 1000, 2000, 3000 U/L) and various concentrations of curcumin (6.25, 12.5, 25 µmol/L) for different time in vitro. The inhibitory ratio was measured by MTT assay. Apoptosis was detected by flow cytometry (FCM). The expression of caspase 6, caspase 8 and caspase 9 in Raji cells treated with IC5025 µmol/L curcumin with IFN-α was examined using Western blot. Results: IFN-α and curcumin could significantly inhibit the growth and induce apoptosis of RAji cells with synergistic effects. They could increase the expression of caspase 6, caspase 8 and caspase 9 in Raji cells in a dose- and time-dependent manner. Conclusion: The combined use of IFN-α and curcumin can inhibit the proliferation of B-NHL Raji cells apparently in vitro. Promotion of the expression of caspase 6, caspase 8, caspase 9 and induction of apoptosis might be one of the important mechanisms.
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