Abstract
Objective To observe inhibitory effects of E2F transcription factor 1 (E2F-1) small interfering RNA (siRNA) on apoptosis of mouse spermatogonial stem cells (SSCs).Methods Percoll density gradient centrifugation and flow cytometry was used to isolate,enrich and purify SSCs.Real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blotting were carried out to investigate the expression of E2F-1,and flow cytometry was ued to analyze the apoptosis rate.Results Compared to non-transfected group and control group,the expression of E2F-1 in experimental group was remarkably down-regulated at mRNA [2-(△△ct),0.32 vs.0.10 and 1.04,P ＜ 0.05] and protein levels (P ＜0.05).Tthe apoptosis rate in experimental group was reduced remarkably (7.69％ vs 23.07％ and 24.19％ ;P＜0.05).Conclusion E2F-1 siRNA could remarkably suppress the apoptosis of SSCs in vitro. Key words: RNA interference; Spermatogonial stem cells; E2F transcription factor 1 ; Apoptosis
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