Inhibitory effects of dunning rat prostate tumor fluid on proliferation of the metastatic MAT-LyLu cell line.

Ilknur Bugan,Seyhan Altun

doi:10.7314/apjcp.2015.16.2.831

Abstract

Tumor fluid accumulation occurs in both human cancer and experimental tumor models. Solid tumors show a tendency to tumor fluid accumulation because of their anatomical and physiological features and this may be influenced by molecular factors. Fluid accumulation in the peri-tumor area also occurs in the Dunning model of rat prostate cancer as the tumor grows. In this study, the effects of tumor fluids that were obtained from Dunning prostate tumor-bearing Copenhagen rats on the strongly metastatic MAT-LyLu cell line were investigatedby examining the cell's migration and tumor fluid's toxicity and the kinetic parameters such as cell proliferation, mitotic index, and labelling index. In this research, tumor fluids were obtained from rats injected with 25105 MAT- LyLu cells and treated with saline solution, and 200 nM tetrodotoxin (TTX), highly specific sodium channel blocker was used. Sterilized tumor fluids were added to medium of MAT-LyLu cells with the proportion of 20% in vitro. Consequently, it was demonstrated that Dunning rat prostate tumor fluid significantly inhibited proliferation (up to 50%), mitotic index, and labeling index of MAT-LyLu cells (up to 75%) (p<0.05) but stimulated the motility of the cells in vitro.

Highlights

Cancer, which begins with the generation of the primary tumor mass from a transformed cell divided in an uncontrolled way, is a multi-step process
It was observed that it increased the survival by decreasing the lung metastasis in the Copenhagen rats which formed prostate cancer with MAT-LyLu cells in vivo (Altun and Djamgoz, 2008; Yildirim et al, 2012). It was the purpose of our study to investigate the effects of the tumor fluids which were obtained from Dunning prostate tumor bearing Copenhagen rats, on the strongly metastatic MAT-LyLu cell line
In the samples prepared from the cells to which the tumor fluids of the animals given both SAL and TTX were applied, cells, which had nuclei similar to apoptotic morphology characterized by the chromatin material densifying as pieces in the periphery of the nucleus and that had not lost its integrity yet, were found (Figure 2)

Summary

Introduction

Cancer, which begins with the generation of the primary tumor mass from a transformed cell divided in an uncontrolled way, is a multi-step process. The effects of tumor fluids that were obtained from Dunning prostate tumor-bearing Copenhagen rats on the strongly metastatic MAT-LyLu cell line were investigatedby examining the cell’s migration and tumor fluid’s toxicity and the kinetic parameters such as cell proliferation, mitotic index, and labelling index.

Results

Conclusion