Inhibitory effects of deguelin on cholangiocarcinoma TFK-1 cells induced angiogenesis

Abstract

Objective To observe and evaluate the inhibitory effects of deguelin on cholangiocarcinoma cells induced human umbilical vein endothelial cells(HUVECs) proliferation, migration and in vivo vascular generation. Methods The effects of deguelin to HUVECs on proliferation were detected by CCK-8.The effects of deguelin to cholangiocarcinoma TFK-1 cells inducing HUVECs on proliferation were detected by Ki-67.The migration and tube formation ability of HUVECs inducing by TFK-1 cells were determined by transwell assay and tube formation assay respectively. The effects of deguelin on TFK-1 cells to angiogenesis in vivo were examined by Matrigel plug assay. Results The deguelin had no effects to HUVECs on proliferation. The expression of Ki-67 of HUVECs treated by TFK-1 cells medium for 24 h was(97.3±0.71)%, comparing to(77.22±1.83)% in 1640 medium, the difference was significant(P<0.01), and(87.86±2.81)% in TFK-1 cells medium with addition of deguelin(P<0.05). The invaded HUVECs that treated by deguelin 1 μmol/L in TFK-1 cells medium for 24 h was(94.67±7.45), comparing with(236.7±13.86) in control group, the difference was significant(P<0.01). The number of new tubules that treated by deguelin 1 μmol/L in TFK-1 cells medium for 24 h was 24.33±3.53, comparing with 60.67±6.12 in control group, the difference was significant(P<0.01). The number of new vessels in Matrigel plug that treated by deguelin group was 9.60±1.36, comparing with 4.49±0.93 in control group, the difference was significant(P<0.05), and the expression of CD31 and VEGF was inhibited by deguelin. Conclusions Deguelin could inhibit the angiogenesis of human umbilical vein endothelial cells that induced by cholangiocarcinoma TFK-1 cells. Key words: Deguelin; Bile duct carcinoma; Angiogenesis; Cell migration